This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with AA. During the reaction, AA in samples or Standard competes with a fixed amount of AA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to AA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of AA in the samples is then determined by comparing the OD of the samples to the standard curve.
AA(Arachidonic Acid) ELISA Kit
£396.00 excluding VAT
| Size | 96T |
|---|---|
| Specificity | This kit recognizes AA in samples. No significant cross-reactivity or interference between AA and analogues was observed. |
| Application | This ELISA kit applies to the in vitro quantitative determination of AA concentrations in Serum, plasma and other biological fluids. |
| Research Areas | Signal transduction |
| Target Species | Universal |
| Assay Type | Competitive |
| Sensitivity | 0.94ng/mL |
| Detection range | 1.56~100ng/mL |
| Sample type | Serum, plasma and other biological fluids |
| Assay length | 2.5h |
| Storage | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
| Citation Link | https://www.sciencedirect.com/science/article/pii/S2090123218301334, http://dx.doi.org/10.1016/j.toxicon.2016.09.011 |
