This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL2. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse COL2. You can calculate the concentration of Mouse COL2 in the samples by comparing the OD of the samples to the standard curve.
Mouse COL2(Collagen Type Ⅱ) ELISA Kit
£396.00 excluding VAT
| Size | 96T |
|---|---|
| Specificity | This kit recognizes Mouse COL2 in samples. No significant cross-reactivity or interference between Mouse COL2 and analogues was observed. |
| Application | This ELISA kit applies to the in vitro quantitative determination of Mouse COL2 concentrations in Serum, plasma and other biological fluids. |
| Research Areas | Cancer, Signal transduction, Stem cells |
| Target Species | Mouse |
| Assay Type | Sandwich |
| Sensitivity | 37.5pg/mL |
| Detection range | 62.5~4000pg/mL |
| Sample type | Serum, plasma and other biological fluids |
| Assay length | 3.5h |
| Storage | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
| Citation Link |
