Fagus Antibody Services

Mouse CST3(Cystatin 3) ELISA Kit

£212.00£300.80 excluding VAT

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 3(CST3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cystatin 3(CST3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin 3(CST3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystatin 3(CST3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Size

48T, 96T

Research Areas

Kidney biomarker, Urology

Species

Mouse

Assay Type

Sandwich

Sensitivity

0.126 ng/mL

Standard

20 ng/mL

Detection range

0.32-20 ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length

3.5h

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