Fagus Antibody Services

Mouse ET-1(Endothelin 1) ELISA Kit

£396.00 excluding VAT

SKU FAS-12938-E Category Tags , , , ,

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ET-1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse ET-1. You can calculate the concentration of Mouse ET-1 in the samples by comparing the OD of the samples to the standard curve.

Size

96T

Specificity

This kit recognizes Mouse ET-1 in samples. No significant cross-reactivity or interference between Mouse ET-1 and analogues was observed.

Application

This ELISA kit applies to the in vitro quantitative determination of Mouse ET-1 concentrations in Serum, plasma and other biological fluids.

Research Areas

Cancer, Cardiovascular, Metabolism, Signal transduction

Target Species

Mouse

Assay Type

Sandwich

Sensitivity

0.47pg/mL

Detection range

0.78~50pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3.5h

Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

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