Fagus Antibody Services

Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)

£105.60£314.40 excluding VAT

SKU FAS-050096-P Category

Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

Size

10µg, 50µg

Active Protein

Active protein

Activity

mmobilized Cynomolgus Siglec-15-His(Cat#PKSQ050096) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 125 ng/ml.

Protein Construction

Recombinant Cynomolgus Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a 6His tag at the C-terminus.

Sequence

Phe20-Thr263

Fusion Tag

C-6His

Accession

A0A2K5UY47

Species

Cynomolgus

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

27.1 kDa

AP Mol Mass

30-40 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, 150mMNaCl, 0.3% Chaps, 5% Trehalose, pH 7.4.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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