Fagus Antibody Services

Recombinant Cynomolgus Signal-Regulatory Protein alpha-1/SIRPA/CD172a (C-6His)

£92.80£277.60 excluding VAT

SKU FAS-050105-P Category

Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation

Size

10µg, 50µg

Active Protein

Active protein

Activity

Immobilized Cynomolgus SIRPA-His(Cat#PKSQ050105) at 10μg/ml (100 μl/well) can bind Anti-Human SIRPA mAb-Fc. The ED50 of Anti-Human SIRPA mAb-Fc is 17.7 ng/ml.

Protein Construction

Recombinant Cynomolgus Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Glu31­Arg369 is expressed with a 6His tag at the C-terminus.

Sequence

Glu31­Arg369

Fusion Tag

C-6His

Accession

I7G9Z7

Species

Cynomolgus

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

37.9 kDa

AP Mol Mass

45-65 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

Related Product