Fagus Antibody Services

Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)

£262.40£532.80 excluding VAT

SKU FAS-050058-P Category Tag

Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant E.coli Lactose operon repressor is produced by our E.coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.

Sequence

Met1-Gln360

Fusion Tag

C-His

Accession

P03023-1

Species

E.coli

Expressed Host

E.coli

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

39.4 kDa

AP Mol Mass

40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.

Research Areas
Reconstitution

Not Applicable

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