β Galactosidase is a lysosomal β Galactosidase that hydrolyzes the terminal β Galactose from Ganglioside and Keratan sulfate. In lysosome, the mature β Galactosidase protein associates with Cathepsin A and Neuraminidase 1 to form the lysosomal multienzyme complex . An alternative splicing at the RNA level of β Galactosidase results a catalytically inactive β Galactosidase that plays an important role in vascular development. Defects of β-galactosidase (GLB1) are the cause of diseases like GM1-gangliosidosis which is a lysosomal storage disease and Morquio Syndrome B that cause patients to have abnormal elastic fibers. More than 100 mutations have been identified for β Galactosidase, which result in different residual activities of the mutant enzymes and a spectrum of symptoms in the two related diseases.
Recombinant Human β-Galactosidase/GLB1 Protein (His Tag)
£231.20 – £566.00 excluding VAT
| Size | 10µg, 50µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | Recombinant Human beta-Galactosidase is produced by our Mammalian expression system and the target gene encoding Leu24-Val677 is expressed with a 6His tag at the C-terminus. |
| Sequence | Leu24-Val677 |
| Fusion Tag | C-6His |
| Accession | P16278 |
| Species | Human |
| Expressed Host | Human Cells |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Mol Mass | 74.6 kDa |
| AP Mol Mass | 90 kDa |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
| Research Areas | Signal Transduction, Cancer, metabolism |
| Reconstitution | Not Applicable |
