Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α; β; and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF; NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF; and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine; which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.
Recombinant Human β-NGF/NGFB Protein (aa 122-241, E. coli)(Active)
£200.00 – £730.00 excluding VAT
| Size | 20µg, 100µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is 1 x 106 IU/mg. |
| Protein Construction | Recombinant Human beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Ala241 is expressed. |
| Sequence | Ser122-Ala241 |
| Fusion Tag | |
| Accession | P01138 |
| Species | Human |
| Expressed Host | E.coli |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 13.4 kDa |
| AP Mol Mass | 14 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.0. |
| Research Areas | Neuroscience |
| Reconstitution | Please refer to the printed manual for detailed information. |
