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Recombinant Human 2B4/CD244 Protein (His Tag)(Active)

£585.60 – £732.00 excluding VAT

SKU FAS-031920-P Category Tag

The CD244 antigen; also known as 2B4; is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production; which is expressed on all NK cells; a subpopulation of T cells; monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor; human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity; induce secretion of IFN-γ and matrix metalloproteinases (MMPs); as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2; CD48; CD58; CD84; Ly-9; and SLAM; 2B4 (CD244) is expressed on all human NK cells; a subpopulation of T cells; basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity; induces secretion of IFN-gamma and matrix metalloproteinases; and NK cell invasiveness.Immune CheckpointImmune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Size

100µg
Active Protein

Active protein
Activity

Measured by its binding ability in a functional ELISA. Immobilized human 2B4 at 2 μg/ml (100 μl/well) can bind human CD48. The EC50 of human CD48 is 0.39 μg/ml.
Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Arg 221) of human 2B4 (NP_057466.1) was expressed; with a polyhistidine tag at the C-terminus.
Sequence

Met 1-Arg 221
Fusion Tag

C-His
Accession

NP_057466.1
Species

Human
Expressed Host

HEK293 Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

23.8 kDa
AP Mol Mass

45-50 kDa
Formulation

Lyophilized from sterile PBS, pH 7.4
Research Areas

immunology, Stem cells
Reconstitution

Please refer to the printed manual for detailed information.

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