Fagus Antibody Services

Recombinant Human 5′-Nucleotidase/5′-NT/CD73 (P21589, C-6His)

£251.20£753.60 excluding VAT

CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5′-nucleotidase family. CD73 is an ecto 5’Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5’Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Size

10µg, 50µg

Active Protein

Active protein

Activity

Immobilized Human CD73-His(Cat#CW84) at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb(Cat#NC034). The ED50 of Anti-Human CD73 mAb(Cat#NC034) is 7.8 ng/ml.

Protein Construction

Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.

Sequence

Trp27-Lys547

Fusion Tag

C-6His

Accession

P21589

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

58.8 kDa

AP Mol Mass

62 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.

Research Areas

Cardiovascular

Reconstitution

Not Applicable

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