ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.
Recombinant Human ACVR2B/ActivinR-IIB Protein (His Tag)(Active)
£363.20 – £454.00 excluding VAT
| Size | 100µg |
|---|---|
| Active Protein | Active protein |
| Activity | 1.Measured by its ability to bind biotinylated Human INHBA-his in functional ELISA.2. Measured by its ability to bind biotinylated Mouse INHBA-his in functional ELISA.3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.3-2 µg/mL in the presence of 10 ng/mL recombinant Activin A. |
| Protein Construction | A DNA sequence encoding the extracellular domain of human ACVR2B (NP_001097.2) (Met 1-Thr 134) was fused with a polyhistidine tag at the C-terminus. |
| Sequence | Met 1-Thr 134 |
| Fusion Tag | C-His |
| Accession | NP_001097.2 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 15 kDa |
| AP Mol Mass | 33-38 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Research Areas | Signal Transduction, Stem cells |
| Reconstitution | Please refer to the printed manual for detailed information. |
