Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Recombinant SARS-CoV-2 N Protein biotinylated(N-6His-Avi)(Active)
£332.00 – £2,940.00 excluding VAT
| Size | 50µg, 500µg |
|---|---|
| Active Protein | Active protein |
| Activity | Immobilized 2019-nCoV NP Antibody (6G9)at 5μg/ml (100 μl/well) can bind 2019-nCoV Nucleocapsid Protein-His-Avi(PKSV030311). The ED50 of PKSV030311 is 92 ng/ml. |
| Protein Construction | Biotinylated 2019-nCoV Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His, Avi tag at the N-terminus. |
| Sequence | Met1-Ala419 |
| Fusion Tag | N-6His |
| Accession | QHD43423.2 |
| Species | SARS-CoV-2 |
| Expressed Host | E.coli |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Stability and Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Mol Mass | 51.3kDa |
| AP Mol Mass | 55-60kDa |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH 7.5 |
| Research Areas | Virus related Protein |
| Reconstitution | Not Applicable |
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