Fagus Antibody Services

Recombinant Rat ASAM Protein (His Tag)(Active)

£585.60£1,191.00 excluding VAT

SKU FAS-030312-P Category Tags ,

Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.

Size

100µg

Active Protein

Active protein

Activity

Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 10E4 cells/well are added to Recombinant Rat ASAM coated plates (12.5 μg/mL with 100 μL/well), 35-70% cells will adhere after 1 hour incubation at 37℃.

Protein Construction

A DNA sequence encoding the rat ASAM (Q8K1G0) (Met1-Met232) was expressed, fused with a polyhistidine tag at the C-terminus.

Sequence

Met1-Met232

Fusion Tag

C-His

Accession

Q8K1G0

Species

Rat

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 85 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

25.6 kDa

AP Mol Mass

37 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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