Fagus Antibody Services

Recombinant Human BCAM Protein (His Tag)

£59.20£214.00 excluding VAT

Basal cell adhesion molecule (BCAM, CD239) is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule (Lu). The ECD of human BCAM contains two Ig-like V-type domains and three Ig-like C2-type domains. It shares 73% aa sequence identity with the ECDs of mouse and rat BCAM. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation. BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. BCAM is upregulated on carcinomas, particularly ovarian, sarcomas, astrocytomas, and melanomas. It may mediate intracellular signaling. It cooperates with Integrins β1 and αVβ3 as an adhesion receptor for Laminins which contain the α5 chain. The Lutheran isoform is aberrantly phosphorylated in erythroid disorders and can enhance Lamininmediated adhesion of erythrocytes to vascular endothelial cells.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human BCAM is produced by our Mammalian expression system and the target gene encoding Glu32-Ala547 is expressed with a 6His tag at the C-terminus.

Sequence

Glu32-Ala547

Fusion Tag

C-His

Accession

P50895

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

57 kDa

AP Mol Mass

72-85 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Research Areas

Cardiovascular, immunology

Reconstitution

Please refer to the printed manual for detailed information.

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