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Recombinant Human BDNF Protein (with pro peptide)(Active)

£103.20 – £375.00 excluding VAT

SKU FAS-033559-P Category Tag

The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

Size

10µg, 50µg
Active Protein

Active protein
Activity

Immobilized Human pro-BDNF at 2ug/ml(100 μl/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human pro-BDNF is 1.4 ug/ml .
Protein Construction

Recombinant Human BDNF is produced by our E.coli expression system and the target gene encoding Ala19-Arg247 is expressed.
Sequence

Ala19-Arg247
Fusion Tag
Accession

P23560
Species

Human
Expressed Host

E.coli
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per μg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

25.9 kDa
AP Mol Mass

28 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
Research Areas

Cardiovascular, Neuroscience, metabolism
Reconstitution

Please refer to the printed manual for detailed information.

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