Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of the alpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
Recombinant Human CA5A/CA-VA Protein (His Tag)(Active)
£576.80 – £721.00 excluding VAT
| Size | 50µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured by its esterase activity. The specific activity is >500 pmoles/min/μg. |
| Protein Construction | A DNA sequence encoding the mature form of human CA5A (NP_001730.1) (Ala 40-Ser 305) was fused with an Met at N-terminus and a polyhistide tag at the C-terminus. |
| Sequence | Ala 40-Ser 305 |
| Fusion Tag | C-His |
| Accession | NP_001730.1 |
| Species | Human |
| Expressed Host | E.coli |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 96 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 31.6 kDa |
| AP Mol Mass | 33 kDa |
| Formulation | Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0 |
| Research Areas | Signal Transduction, Tags & Cell Markers, metabolism |
| Reconstitution | Please refer to the printed manual for detailed information. |
