Carboxypeptidase A1 （CPA1）is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.
Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)(Active)
£693.60 – £867.00 excluding VAT
| Size | 50µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >3, 500 pmoles/min/μg. |
| Protein Construction | A DNA sequence encoding the human CPA1 precursor (NP_001859.1) (Met 1-Tyr 419) was expressed with a C-terminal polyhistidine tag. |
| Sequence | Met 1-Tyr 419 |
| Fusion Tag | C-His |
| Accession | NP_001859.1 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 47 kDa |
| AP Mol Mass | 43 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Research Areas | Signal Transduction, Cell biology, metabolism |
| Reconstitution | Please refer to the printed manual for detailed information. |
Related Product
Related products
-
-
-
-
-
-
Protein
Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD (C-mFc-6His tag)(Active)
£180.00 – £875.00 excluding VAT -
Protein
Recombinant ZIKV (strain Zika SPH2016) Stem/anchor domain of flavivirus envelope glycoprotein E protein (Fc Tag)
£540.00 – £855.00 excluding VAT -
-
-