Fagus Antibody Services

Recombinant Human Carboxypeptidase E/CPE Protein (Fc Tag)

£693.60£867.00 excluding VAT

Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type I I diabetes due to impaired glucose clearance and insulin resistance.

Size

50µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was expressed with the C-terminal fused Fc region of human IgG1.

Sequence

Met 1-Ser 453

Fusion Tag

C-Fc

Accession

NP_001864.1

Species

Human

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 85 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

74.6 kDa

AP Mol Mass

85-90 kDa

Formulation

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5

Research Areas

Signal Transduction, Neuroscience, Cancer, metabolism

Reconstitution

Please refer to the printed manual for detailed information.

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