Cathepsin D (CTSD); a well known lysosomal aspartyl protease and belongs to the peptidase C1 family; which is a normal and major component of lysosomes; and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments; other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins; the generation of bioactive proteins; antigen processing; etc. Among different role in cell physiology; a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition; CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin; a potent endogenous inhibitor of angiogenesis; suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.
Recombinant Human Cathepsin D/CTSD Protein (His Tag)(Active)
£693.60 – £6,600.00 excluding VAT
| Size | 50µg, 1mg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured by its ability to bind biotinylated human CTSS-His in a functional ELISA. |
| Protein Construction | A DNA sequence encoding the pro form of human CTSD (P07339) (Met 1-Leu 412) was fused with a polyhistidine tag at the C-terminus. |
| Sequence | Met 1-Leu 412 |
| Fusion Tag | C-His |
| Accession | P07339 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 44 kDa |
| AP Mol Mass | 40-110 kDa |
| Formulation | Lyophilized from sterile 25mM MES, 150mM NaCl, pH 6.5 |
| Research Areas | Signal Transduction, Cell biology, Neuroscience, Cancer |
| Reconstitution | Please refer to the printed manual for detailed information. |
