The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer’s disease, providing strong evidence for the protein’s association with the Alzheimer’s disease process. CD10 is also associated with other biochemical processes.
Recombinant Human CD10/Neprilysin Protein (Fc Tag)(Active)
£576.80 – £721.00 excluding VAT
Size | 50µg |
---|---|
Active Protein | Active protein |
Activity | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH, (R&D Systems, Catalog # ES005). The specific activity is >1000 pmoles/min/μg. |
Protein Construction | A DNA sequence encoding the extracellular domain (Tyr 52-Trp 750) of human membrane metallo-endopeptidase (NP_000893.2) was fused with the Fc region of human IgG1 at the N-terminus. |
Sequence | Tyr 52-Trp 750 |
Fusion Tag | N-Fc |
Accession | NP_000893.2 |
Species | Human |
Expressed Host | HEK293 Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 106 kDa |
AP Mol Mass | 120-130 kDa |
Formulation | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 |
Research Areas | Cancer, immunology, Stem cells |
Reconstitution | Please refer to the printed manual for detailed information. |
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