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Recombinant Human CD3 epsilon/CD3E (C-mFC)

£105.60 – £314.40 excluding VAT

SKU FAS-033869-P Category Tag

T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Size

10µg, 50µg
Active Protein

Active protein
Activity

Immobilized  Human  CD3E-mFc(Cat#C00E) at 10μg/ml (100 μl/well) can bind Human Anti-CD3(Cat#GMP-A018).  The ED50 of Human CD3E-mFc(Cat#C00E) is 9.4 ng/ml.
Protein Construction

Recombinant Human T-Cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Gln22-Thr48 is expressed with a mFc tag at the C-terminus.
Sequence

Gln22-Thr48
Fusion Tag

C-mFC
Accession

P07766
Species

Human
Expressed Host

Human Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

28.5 kDa
AP Mol Mass

32-40 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Research Areas

immunology, Stem cells
Reconstitution

Please refer to the printed manual for detailed information.

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