TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Recombinant Rat TrkB/NTRK2 Protein (His Tag)(Active)
£292.00 – £1,688.00 excluding VAT
Size | 20µg |
---|---|
Active Protein | Active protein |
Activity | Measured by its binding ability in a functional ELISA.Immobilized rat NTRK2-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse BDNF, The EC50 of biotinylated mouse BDNF is 12.2-28.6 ng/ml. |
Protein Construction | A DNA sequence encoding the rat NTRK2 (Q63604-Isoform T1) (Met1-His429) was expressed with a polyhistidine tag at the C-terminus. |
Sequence | Met1-His429 |
Fusion Tag | C-His |
Accession | Q63604-Isoform T1 |
Species | Rat |
Expressed Host | HEK293 Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 47.1 kDa |
AP Mol Mass | 67 kDa |
Formulation | Lyophilized from sterile PBS, pH 7.4 |
Research Areas | |
Reconstitution | Please refer to the printed manual for detailed information. |
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