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Recombinant Human CD40/TNFRSF5 Protein (His Tag)(Active)

£49.60 – £167.00 excluding VAT

SKU FAS-033723-P Category Tag

CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells; follicular dendritic cells; dendritic cells; activated monocytes; macrophages; endothelial cells; vascular smooth muscle cells; and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand (CD40L) leads to aggregation of CD40 molecules; which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins (adaptor proteins) have been identified to serves as mediators of the signal transduction. CD40 plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching; memory B cell development; and germinal center formation.

Size

10µg, 50µg
Active Protein

Active protein
Activity

Immobilized Human CD40L(Cat: PKSH033725) at 5μg/ml(100 μl/well) can bind Human CD40-His. The ED50 of Human CD40-His is 44.004 ug/ml .
Protein Construction

Recombinant Human CD40 is produced by our Mammalian expression system and the target gene encoding Glu21-Arg193 is expressed with a 6His tag at the C-terminus.
Sequence

Glu21-Arg193
Fusion Tag

C-His
Accession

P25942
Species

Human
Expressed Host

Human Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per μg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

20.2 kDa
AP Mol Mass

28 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Research Areas

Cardiovascular, immunology, Stem cells
Reconstitution

Please refer to the printed manual for detailed information.

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