Fagus Antibody Services

Recombinant Human CDC42 protein(His tag)

£38.40£92.00 excluding VAT

SKU FAS-500077-P Category Tag

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase.Regulates cell migration.In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections.Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation.Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling.Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.

Size

20µg, 100µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding theHuman CDC42 protein (P60953-2) (Met1-Cys188) was expressed with a N-His.

Sequence

Met1-Cys188

Fusion Tag

His

Accession

P60953-2

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

20.9 kDa

AP Mol Mass

24 kDa

Formulation

Lyophilized from sterile PBS, pH 7.5, 5% trehalose, 5% mannitol, 0.01% tween-80.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

Related Product