CLEC1B, also known as CLEC2, is a C-type lectin-like receptor expressed in myeloid cells and NK cells. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC2 contains 1 C-type lectin domain and is expressed preferentially in the liver. It acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.
Recombinant Human CLEC1B/CLEC2 Protein (His Tag)(Active)
£752.00 – £6,128.00 excluding VAT
| Size | 100µg, 1mg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized human Podoplanin at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.71 μg/mL. |
| Protein Construction | A DNA sequence encoding the human CLEC1B (NP_057593.3) extracellular domain (Gln 58-Pro 229) with a N-terminal polyhistidine tag was expressed. |
| Sequence | Gln 58-Pro 229 |
| Fusion Tag | N-His |
| Accession | NP_057593.3 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 76 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 22.7 kDa |
| AP Mol Mass | 35-38 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Research Areas | immunology |
| Reconstitution | Please refer to the printed manual for detailed information. |
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