Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Recombinant Human coronavirus (HCoV-NL63) Nucleoprotein / NP Protein (His Tag)
£884.00 excluding VAT
| Size | 100µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | A DNA sequence encoding the Human coronavirus (HCoV-NL63) Nucleoprotein (YP_003771.1) (Met1-His377) was expressed with a polyhistide tag at the N-terminus. |
| Sequence | Met1-His377 |
| Fusion Tag | N-His |
| Accession | YP_003771.1 |
| Species | HCoV-NL63 |
| Expressed Host | E.coli |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 43.21kDa. |
| AP Mol Mass | |
| Formulation | Lyophilized from sterile 40mM PB 500mM NaCl, pH 7.4 Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Research Areas | Virus related Protein |
| Reconstitution | Please refer to the printed manual for detailed information. |
