Fagus Antibody Services

Recombinant Human CRABP2 Protein (His Tag)

£388.80£486.00 excluding VAT

SKU FAS-031210-P Category Tag

Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.

Size

100µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human CRABP2 (NP_001869.1) (Pro2-Glu 138) was expressed, with a polyhistide tag at the N-terminus.

Sequence

Pro2-Glu 138

Fusion Tag

N-His

Accession

NP_001869.1

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 96 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

16.5 kDa

AP Mol Mass

16.5 kDa

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0

Research Areas

Signal Transduction, Cancer, epigenetics and nuclear signal

Reconstitution

Please refer to the printed manual for detailed information.

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