Cytotoxic T-lymphocyte protein 4; is a single-pass type I membrane protein. It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.
Recombinant Human CTLA4 Protein (Fc & Avi Tag)(Active)
£273.60 – £739.20 excluding VAT
Size | 20µg, 100µg |
---|---|
Active Protein | Active protein |
Activity | Immobilized Human CTLA-4-Fc-Avi at 2μg/ml(100 μl/well) can bind Human B7-2-6His(Cat: PKSH033722). The ED50 of Human CTLA-4-Fc-Avi is 1.98 ug/mL |
Protein Construction | Biotinylated Recombinant Human CTLA4 is produced by our Mammalianexpression system and the target gene encoding Lys36-Asp161 is expressed with a Fc; Avi tag at the C-terminus. |
Sequence | Lys36-Asp161 |
Fusion Tag | C-Fc-Avi |
Accession | P16410 |
Species | Human |
Expressed Host | Human Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 41.5 kDa |
AP Mol Mass | 57-60 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Research Areas | immunology, Stem cells |
Reconstitution | Please refer to the printed manual for detailed information. |
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