Fagus Antibody Services

Recombinant Human CTLA4 Protein (Fc & Avi Tag)(Active)

£273.60£739.20 excluding VAT

Cytotoxic T-lymphocyte protein 4; is a single-pass type I membrane protein. It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

Size

20µg, 100µg

Active Protein

Active protein

Activity

Immobilized Human CTLA-4-Fc-Avi at 2μg/ml(100 μl/well) can bind Human B7-2-6His(Cat: PKSH033722). The ED50 of Human CTLA-4-Fc-Avi is 1.98 ug/mL

Protein Construction

Biotinylated Recombinant Human CTLA4 is produced by our Mammalianexpression system and the target gene encoding Lys36-Asp161 is expressed with a Fc; Avi tag at the C-terminus.

Sequence

Lys36-Asp161

Fusion Tag

C-Fc-Avi

Accession

P16410

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

41.5 kDa

AP Mol Mass

57-60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Research Areas

immunology, Stem cells

Reconstitution

Please refer to the printed manual for detailed information.

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