Fagus Antibody Services

Recombinant Human CXADR/CAR Protein (His & Fc Tag)(Active)

£585.60£732.00 excluding VAT

CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I  transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.

Size

100µg

Active Protein

Active protein

Activity

Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10E4 cells/well are added to CXADR coated plates (4 μg/ml and 100 μl/well), approximately 20%-40% will adhere specifically after 60 minutes at 37℃.

Protein Construction

A DNA sequence encoding the human CXADR (NP_001329.1) extracellular domain (Met 1-Gly 237) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Sequence

Met 1-Gly 237

Fusion Tag

C-His & Fc

Accession

NP_001329.1

Species

Human

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 92 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

52 kDa

AP Mol Mass

60-65 kDa

Formulation

Lyophilized from sterile PBS, 20% Glycerol, pH 7.4, 5% Trehalose, 5% Mannitol, 0, 01% Tween-80.

Research Areas

Signal Transduction, Microbiology

Reconstitution

Please refer to the printed manual for detailed information.

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