Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. In mammals, four Notch homologs (Notch 1 to4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 shares 86% amino acid sequence identity. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteinerich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands result in reciprocal regulated intramembrane proteolysis (RIP). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ γ secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively.
Recombinant Human Delta-like Protein 4/DLL4 (C-Fc)
£74.40 – £221.60 excluding VAT
Size | 10µg, 50µg |
---|---|
Active Protein | |
Activity | |
Protein Construction | Recombinant Human Delta-like Protein 4 is produced by our Mammalian expression system and the target gene encoding Ser27-Pro524 is expressed with a Fc tag at the C-terminus. |
Sequence | Ser27-Pro524 |
Fusion Tag | C-Fc |
Accession | Q9NR61 |
Species | Human |
Expressed Host | Human Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 81.2 kDa |
AP Mol Mass | 85-100 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Research Areas | Cell biology, Neuroscience, epigenetics and nuclear signal |
Reconstitution | Please refer to the printed manual for detailed information. |
Related Product
Related products
-
Protein
Recombinant ZIKV (strain Zika SPH2015) NS5 protein (His Tag)
£363.20 – £855.00 excluding VAT