Fagus Antibody Services

Recombinant Human Delta-like Protein 4/DLL4 (C-Fc)

£74.40£221.60 excluding VAT

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. In mammals, four Notch homologs (Notch 1 to4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 shares 86% amino acid sequence identity. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteinerich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands result in reciprocal regulated intramembrane proteolysis (RIP). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ γ secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human Delta-like Protein 4 is produced by our Mammalian expression system and the target gene encoding Ser27-Pro524 is expressed with a Fc tag at the C-terminus.

Sequence

Ser27-Pro524

Fusion Tag

C-Fc

Accession

Q9NR61

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

81.2 kDa

AP Mol Mass

85-100 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Research Areas

Cell biology, Neuroscience, epigenetics and nuclear signal

Reconstitution

Please refer to the printed manual for detailed information.

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