Fagus Antibody Services

Recombinant Human DNASE1/Deoxyribonuclease I/DNL1 Protein (His Tag)

£684.00£855.00 excluding VAT

SKU FAS-030557-P Category Tag

DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5′-phosphate-terminated polynucleotides with a free hydroxyl group on position 3′, on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.

Size

100µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human DNASE1 (P24855) (Met1-Lys282) was expressed with a polyhistidine tag at the C-terminus.

Sequence

Met 1-Lys282

Fusion Tag

C-His

Accession

P24855

Species

Human

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 85 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

30.7 kDa

AP Mol Mass

37 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas

Cell biology, Cancer, epigenetics and nuclear signal, metabolism

Reconstitution

Please refer to the printed manual for detailed information.

Related Product