Fagus Antibody Services

Recombinant Human ECH1 Protein

£220.00£525.00 excluding VAT

Human delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase(ECH1) is a member of the hydratase/isomerase superfamily and contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The rat orthologlocalizes to the matrix of both the peroxisome and mitochondria.It can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 plays an important role in the auxiliary step of the fatty acid beta-oxidation pathway.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human Delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial is produced by our E.coli expression system and the target gene encoding Thr34-Leu328 is expressed with a 6His tag at the N-terminus.

Sequence

Thr34-Leu328

Fusion Tag

N-6His

Accession

Q13011

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

34.5 kDa

AP Mol Mass

30-35 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0.

Research Areas

Signal Transduction, Tags & Cell Markers, Cardiovascular, Cancer, metabolism

Reconstitution

Not Applicable

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