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Recombinant Human EGF Protein (Active)

£200.00 – £730.00 excluding VAT

SKU FAS-033687-P Category Tag

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

Size

20µg, 100µg
Active Protein

Active protein
Activity

Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 106 IU/mg.
Protein Construction

Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.
Sequence

Asn971-Arg1023
Fusion Tag
Accession

P01133
Species

Human
Expressed Host

E.coli
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per μg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

6.2 kDa
AP Mol Mass

11 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.
Research Areas

Signal Transduction, Cell biology, Cancer
Reconstitution

Please refer to the printed manual for detailed information.

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