The EGFR subfamily of receptor tyrosine kinases is composed of EGFR; ErbB2; ErbB3 and ErbB4. The EGFR shares 43% – 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2; resulting in cell signaling; heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF; amphiregulin; TGF-alpha; betacellulin; epiregulin; HB-EGF; epigen; and so on. Its signaling regulates multiple biological functions including cell proliferation; differentiation; motility; and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.
Recombinant Human EGFR/ErbB1 Protein (His & Avi Tag)
£273.60 – £739.20 excluding VAT
Size | 20µg, 100µg |
---|---|
Active Protein | |
Activity | |
Protein Construction | Biotinylated Recombinant Human Epidermal Growth Factor Receptor/Receptor Tyrosine Protein KinaseErbB1 is produced by our Mammalian expression system and the target gene encoding Leu25-Val30Gly&Asn298-Ser645 is expressed with a 6His; Avi tag at the C-terminus. |
Sequence | Leu25-Val30Gly&Asn298-Ser645 |
Fusion Tag | C-His-Avi |
Accession | P00533 |
Species | Human |
Expressed Host | Human Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 41.5 kDa |
AP Mol Mass | 60-90 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Research Areas | Signal Transduction, Cancer |
Reconstitution | Please refer to the printed manual for detailed information. |
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