Fagus Antibody Services

Recombinant Human EGFR/ErbB1 Protein (His & Avi Tag)

£273.60£739.20 excluding VAT

The EGFR subfamily of receptor tyrosine kinases is composed of EGFR; ErbB2; ErbB3 and ErbB4. The EGFR shares 43% – 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2; resulting in cell signaling; heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF; amphiregulin; TGF-alpha; betacellulin; epiregulin; HB-EGF; epigen; and so on. Its signaling regulates multiple biological functions including cell proliferation; differentiation; motility; and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.

Size

20µg, 100µg

Active Protein
Activity
Protein Construction

Biotinylated Recombinant Human Epidermal Growth Factor Receptor/Receptor Tyrosine Protein KinaseErbB1 is produced by our Mammalian expression system and the target gene encoding Leu25-Val30Gly&Asn298-Ser645 is expressed with a 6His; Avi tag at the C-terminus.

Sequence

Leu25-Val30Gly&Asn298-Ser645

Fusion Tag

C-His-Avi

Accession

P00533

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

41.5 kDa

AP Mol Mass

60-90 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Research Areas

Signal Transduction, Cancer

Reconstitution

Please refer to the printed manual for detailed information.

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