Fagus Antibody Services

Recombinant Human EphB2 Protein (His Tag)(Active)

£585.60£732.00 excluding VAT

SKU FAS-031441-P Category Tag

Ephrin type-B receptor 2; also known as EphB2; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses; suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors; but does not require the kinase activity of EphB.

Size

100µg

Active Protein

Active protein

Activity

Measured by its ability to bind recombinant human EphrinB2 / Fc chimera in a functional ELISA.

Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Leu 543) of human EphB2 (NP_059145.2) was fused with a polyhistidine tag at the C-terminus.

Sequence

Met 1-Leu 543

Fusion Tag

C-His

Accession

NP_059145.2

Species

Human

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 98 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

59.7 kDa

AP Mol Mass

70-75 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas

Signal Transduction, Cardiovascular, Neuroscience, Cancer, Stem cells

Reconstitution

Please refer to the printed manual for detailed information.

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