Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.
Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155)(Q65I,C96S,N111G)
£68.80 – £178.00 excluding VAT
Size | 10µg, 50µg |
---|---|
Active Protein | |
Activity | |
Protein Construction | Recombinant Human FGF-2(Q65I;C96S;N111G) is produced by our E.coli expression system and the target gene encoding Met1-Ser155 is expressed. |
Sequence | Met1-Ser155 |
Fusion Tag | |
Accession | P09038-2 |
Species | Human |
Expressed Host | E.coli |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 17.2 kDa |
AP Mol Mass | 16 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH7.5. |
Research Areas | |
Reconstitution | Please refer to the printed manual for detailed information. |
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