Growth Arrest and DNA Damage-Inducible Protein GADD45 α (GADD45A) is a member of the GADD45 family. GADD45A can be induced by UV irradiation; X-rays; growth arrest and alkylating agents; of which can be mediated by some kinases other than PKC. GADD45A can interact with MAPK14; GADD45GIP1; PCNA. In T-cells; GADD45A functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. GADD45A may affect PCNA interaction with some cell division protein kinase complexes; stimulating DNA excision repair in vitro and inhibits entry of cells into S phase.
Recombinant Human GADD45A/DDIT-1 Protein (His Tag)
£142.40 – £523.00 excluding VAT
| Size | 10µg, 50µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | Recombinant Human GADD45A alpha is produced by our E.coli expression system and the target gene encoding Met1-Arg165 is expressed with a 6His tag at the N-terminus. |
| Sequence | Met1-Arg165 |
| Fusion Tag | N-His |
| Accession | P24522 |
| Species | Human |
| Expressed Host | E.coli |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 20.5 kDa |
| AP Mol Mass | 18 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Research Areas | epigenetics and nuclear signal |
| Reconstitution | Please refer to the printed manual for detailed information. |
