Fagus Antibody Services

Recombinant Human GAPDH protein (His Tag)

£79.20£360.00 excluding VAT

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3′-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Size

20µg, 100µg

Active Protein

Active protein

Activity

Immunogen(E-AB-40337)

Protein Construction

A DNA sequence encoding the Human GAPDH (P04406) (1M-333E) was expressed with a polyhistidine tag at the N-terminus.

Sequence

1M-333E

Fusion Tag

N/C-His

Accession

P04406

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 90 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

37.1kDa

AP Mol Mass

34-37kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas

Signal Transduction, Cancer, Isotypeloading control, metabolism

Reconstitution

Please refer to the printed manual for detailed information.

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