Fagus Antibody Services

Recombinant Human Glutaminyl cyclase/QPCT Protein (His Tag)

£693.60£867.00 excluding VAT

SKU FAS-030564-P Category Tag

Glutaminyl cyclase, also known as QPCT, can promote the N-terminal cyclization reaction of N-terminal pyroglutamate(pGlu). The pGlu formation from its glutaminyl precursor is required in the maturation of numerous bioactive peptides, while the aberrant formation of pGlu may be related to several pathological processes, such as osteoporosis and amyloidotic diseases. Glutaminyl cyclase’s structure reveals an alpha/beta scaffold akin to that of two-zinc exopeptidases but with several insertions and deletions, particularly in the active-site region. Glutaminyl cyclase’s amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. It is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.

Size

50µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human QPCT (Q16769-1) (Ala33-Leu361) was fused with a polyhistide tag at the N-terminus.

Sequence

Ala33-Leu361

Fusion Tag

N-His

Accession

Q16769-1

Species

Human

Expressed Host

Baculovirus-Insect Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

39.7 kDa

AP Mol Mass

38 kDa

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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