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Recombinant Human IL6RA/CD126 Protein (His Tag)(Active)

£684.00 – £5,564.00 excluding VAT

SKU FAS-031632-P Category Tag

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called ‘trans-signalling’ mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Size

100µg, 1mg
Active Protein

Active protein
Activity

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/mL (100μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/ml.2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/ml.
Protein Construction

A DNA sequence encoding the extracellular domain of human IL6R (NP_000556.1) corresponding to amino acid (Met 1-Pro 365) was expressed with a C-terminal polyhistidine tag.
Sequence

Met 1-Pro 365
Fusion Tag

C-His
Accession

NP_000556.1
Species

Human
Expressed Host

HEK293 Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 90 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg of the protein as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

40 kDa
AP Mol Mass
Formulation

Lyophilized from sterile PBS, pH 7.4
Research Areas

immunology, Stem cells
Reconstitution

Please refer to the printed manual for detailed information.

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