Fagus Antibody Services

Recombinant SARS-CoV-2 N Protein (P80R)(His Tag)

£573.60£1,105.00 excluding VAT

SKU FAS-030373-P Category

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Size

100µg

Active Protein
Activity

Testing in progress

Protein Construction

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid (YP_009724397.2, with mutation P80R) (Met1-Ala419) was expressed with a polyhistidine tag at the N-terminus. The mutation were identified in the SARS-CoV-2 variant (known as variant P.1) which emerged in the Brazil.

Sequence

Met1-Ala419

Fusion Tag

N-His

Accession

YP_009724397.2

Species

SARS-CoV-2

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

46.7 kDa

AP Mol Mass
Formulation

Lyophilized from sterile 50 mM PB, 500 mM NaCl, pH 7.0.

Research Areas

Virus related Protein

Reconstitution

Please refer to the printed manual for detailed information.

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