IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.
Recombinant Human Interleukin-12/IL-12 Protein (Active)
£142.40 – £523.00 excluding VAT
Size | 10µg, 50µg |
---|---|
Active Protein | Active protein |
Activity | Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.1-0.5ng/ml. |
Protein Construction | Recombinant Human Interleukin-12 is produced by our Mammalian expression system and the target gene encoding Arg23-Ser219&Ile23-Ser328 is expressed. |
Sequence | Arg23-Ser219&Ile23-Ser328 |
Fusion Tag | |
Accession | NP_002178.2 |
Species | Human |
Expressed Host | Human Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 22.5&34.7 kDa |
AP Mol Mass | 40-50 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Research Areas | Microbiology, Cancer, immunology |
Reconstitution | Please refer to the printed manual for detailed information. |