Fagus Antibody Services

Recombinant Human Interleukin-23/IL-23 (C-6His)

£221.60£650.40 excluding VAT

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.

Size

10µg, 50µg

Active Protein

Active protein

Activity

Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells.The ED50 for this effect is 70-210 ng/ml. 

Protein Construction

Recombinant Human Interleukin-23 is produced by our Mammalian expression system and the target gene encoding Arg20-Pro189&Ile23-Ser328 is expressed with a 6His tag at the C-terminus.

Sequence

Arg20-Pro189&Ile23-Ser328

Fusion Tag

C-6His

Accession

Q9NPF7&P29460

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

19.7&34.8 kDa

AP Mol Mass

19-21&40-60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Research Areas

Microbiology, Cancer, immunology

Reconstitution

Please refer to the printed manual for detailed information.

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