Junctional adhesion molecule-A (JAM-A); also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321); is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells; as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1; involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily; JAM-A protein involves in platelet adhesion; secretion and aggregation; and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition; it may be a potential therapeutic target for breast cancer.
Recombinant Human JAM-A/F11R Protein (His Tag)(Active)
£585.60 – £732.00 excluding VAT
| Size | 100µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to JAM-A-Fc coated plates (2.5μg/mL; 100 μL/well)in the presence of 20 ng/mL PMA; approximately 30-40% will adhere after 30 minutes at 37℃. |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Ala 242) of human JAM-A (NP_058642.1) precursor was expressed with a polyhistidine tag at the C-terminus. |
| Sequence | Met 1-Ala 242 |
| Fusion Tag | C-His |
| Accession | NP_058642.1 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 25 kDa |
| AP Mol Mass | 28-32 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Research Areas | Signal Transduction, Cardiovascular |
| Reconstitution | Please refer to the printed manual for detailed information. |
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