Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
Recombinant Human KEAP1/INRF2 Protein (His & GST Tag)
£585.60 – £732.00 excluding VAT
| Size | 100µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | A DNA sequence encoding the human KEAP1 (NP_036421.2) (Gln2-Cys624) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Sequence | Gln2-Cys624 |
| Fusion Tag | N-His & GST |
| Accession | NP_036421.2 |
| Species | Human |
| Expressed Host | Baculovirus-Insect Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 97.5 kDa |
| AP Mol Mass | 109 kDa |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4 |
| Research Areas | epigenetics and nuclear signal |
| Reconstitution | Please refer to the printed manual for detailed information. |
