Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’s involved in growth promotion and cell differentiation of different types of target cells; influence on bone metabolism; cachexia; neural development; embryogenesis and inflammation. LIF has potent proinflammatory property; being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells; hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors; the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities; LIF is involved in the pathogenic events and development of many diseases of various origin.
Recombinant Human LIF Protein (Fc Tag)(Active)
£753.60 – £942.00 excluding VAT
| Size | 100µg |
|---|---|
| Active Protein | Active protein |
| Activity | 1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 ng/ml.2. Measured by its ability to bind human LIFR-His in a functional ELISA. |
| Protein Construction | A DNA sequence encoding the human LIF (P15018)(Met1-Phe202) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Sequence | Met 1-Phe202 |
| Fusion Tag | C-Fc |
| Accession | P15018 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 46.7 kDa |
| AP Mol Mass | 63 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Research Areas | Cell biology, Neuroscience, Cancer, epigenetics and nuclear signal, immunology |
| Reconstitution | Please refer to the printed manual for detailed information. |
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