Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independant growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.
Recombinant Human MAP1D Protein (His Tag)(Active)
£585.60 – £732.00 excluding VAT
| Size | 50µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC, R&D Systems, Catalog#ES017. The resulting GP-AMC is cleaved by human DPPIV/CD26, R&D Systems, Catalog#1180SE. The specific activity is >30 pmoles/min/μg. |
| Protein Construction | A DNA sequence encoding the human MAP1D (NP_954697.1) (Arg 44-Ala 335) was expressed, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus. |
| Sequence | Arg 44-Ala 335 |
| Fusion Tag | C-His |
| Accession | NP_954697.1 |
| Species | Human |
| Expressed Host | Baculovirus-Insect Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 92 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 33.4 kDa |
| AP Mol Mass | 36 kDa |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0 |
| Research Areas | Signal Transduction, epigenetics and nuclear signal |
| Reconstitution | Please refer to the printed manual for detailed information. |
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