MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.
Recombinant Human MGMT Protein (His Tag)
£210.40 – £489.00 excluding VAT
| Size | 10µg, 50µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | Recombinant Human Methylated-DNA–protein-cysteine methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus. |
| Sequence | Met 1-Asn207 |
| Fusion Tag | N-6His |
| Accession | P16455 |
| Species | Human |
| Expressed Host | E.coli |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Mol Mass | 23.8 kDa |
| AP Mol Mass | 25 kDa |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mMTrisHCl, 1mM EDTA, pH 8.0 . |
| Research Areas | epigenetics and nuclear signal |
| Reconstitution | Not Applicable |
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