Fagus Antibody Services

Recombinant Human MMP-2 Protein (His Tag)

£142.40£523.00 excluding VAT

72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is encoded by the MMP2 gene.It belongs to the matrix metalloproteinase (MMP) family. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis; differentiation; angiogenesis and tissue remodeling; as well as pathological processes including inflammation; arthritis; cardiovascular diseases; pulmonary diseases and tumor invasion. MMP-2 is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature; angiogenesis; tissue repair; tumor invasion; inflammation; atherosclerotic plaque rupture; as well as degrading extracellular matrix proteins. MMP-2 can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. MMP-2 cleaves KISS at a Gly-|-Leu bond and appears to have a role in myocardial cell death pathways.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human Matrix Metalloproteinase-2 is produced by our Mammalian expression system and the target gene encoding Ala30-Cys660 is expressed with a 6His tag at the C-terminus.

Sequence

Ala30-Cys660

Fusion Tag

C-6His

Accession

NP_004521.1

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 90 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

72.01 kDa

AP Mol Mass

69 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 7.5.

Research Areas

Cell biology, Cardiovascular, Cancer, metabolism

Reconstitution

Please refer to the printed manual for detailed information.

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